TLR expression profile of human gingival margin-derived stem progenitor cells

BACKGROUND: Gingival margin-derived stem/progenitor cells (G-MSCs) show remarkable periodontal regenerative potential in vivo. During regeneration, G-MSCs may interact with their inflammatory environment via toll-likereceptors (TLRs). The present study aimed to depict the G-MSCs TLRs expression profile. MATERIAL AND METHODS: Cells were isolated from free gingival margins, STRO-1-immunomagnetically sorted and seeded to obtain single colony forming units (CFUs). G-MSCs were characterized for CD14, CD34, CD45, CD73, CD90, CD105, CD146 and STRO-1 expression, and for multilineage differentiation potential. Following G-MSCsˇŻ incubation in basic or inflammatory medium (IL-1¦Â, IFN-¦Ă, IFN-¦Á, TNF-¦Á) a TLR expression profile was generated. RESULTS: G-MSCs showed all stem/progenitor cellsˇŻ characteristics. In basic medium G-MSCs expressed TLRs 1, 2, 3, 4, 5, 6, 7, and 10. The inflammatory medium significantly up-regulated TLRs 1, 2, 4, 5, 7 and 10 and diminished TLR 6 (pˇÜ0.05, Wilcoxon-Signed-Ranks-Test). CONCLUSIONS: The current study describes for the first time the distinctive TLRs expression profile of G-MSCs under uninflamed and inflamed conditions

A functional acrylic periodontal veneer (case report)

Acrylic periodontal veneers were recommended to improve - aesthetics following periodontal surgery, especially when there is considerable apical movement of the gingival margin with subsequent exposure of roots (L'Estrange and strahan 1970). The present report is a case of a young sudanese lady, 30 years old, who presented with severe gingival recession on the lingual surface of 21/12 with subsequent exposure of the roots as a result of inflammatory periodontal disease. The exposed roots were sharp enough to cause a traumatic ulcer at the tip of the tongue. Healing of the ulcer was only attainable following construction of an acrylic periodontal veneer to cover the sharp roots and was placed on the lingual surface of 21/12. The patients acceptability to the veneer was excellent and encouraging.